Figure 3.

LINC00365 inhibits proliferation through regulating SCGB2A1 in gastric cancer cells. (A, B) BGC-823 and MGC-803 cells were transfected with LINC00365 overexpression plasmid or empty vector. Cell viability was analyzed by the MTT assay. Data are presented as means ± SD; *P < 0.05. (C) BGC-823 and MGC-803 cells were treated as in A. Cells were stained after 48 h with EdU and Hoechst 33342 and observed using a fluorescence microscope (scale bar, 50 µm). (D–F) Clone formation assays in BGC-823 and MGC-803 cells transfected with SCGB2A1 overexpression plasmid or empty vector. Data are presented as means ± SD; *P < 0.05. (G) Transfected BGC-823 and MGC-803 cells were stained with PI and Annexin V-FITC. Positively stained cells were detected by flow cytometry.