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. 2020 Jan 19;2020:1953242. doi: 10.1155/2020/1953242

Figure 4.

Figure 4

mmu-miR-146b-5p targets Tbc1d14. (a) Tbc1d14 mRNA levels in cells transfected with miR-NC mimic and miR-146b mimic. RAW264.7 were transfected with miR-146b mimic (100 nM), and the Tbc1d14 mRNA levels were measured at 24 h after transfection by qRT-PCR assays. Control (con) cells were transfected with miR-NC mimic. (b, c) Mutation of Tbc1d14 3′UTR. The binding sites of miR-146b in the Tbc1d14 3′UTR (AGUUCUCC) were predicted by bioinformatics software. AGUUCUC (the binding sites) were mutated into CUGGAGU (red). (d) The miR-146b-5p binding sites in mouse Tbc1d14-3′UTR mediates the repression of luciferase activities in cells. Data are mean ± SD from three independent experiments. ∗p < 0.05; ∗∗p < 0.01.