Figure 3. Tumor-Specific Expression of VISTA Promotes Tumor Onset.

(A) The mouse melanoma cell line, D4M UV2, was engineered to express a V5-tagged VISTA, and expression was verified by western blot.

(B) As for (A), except that expression was verified by flow cytometry.

(C) In vitro cell growth of D4M UV2 cells expressing VISTA was evaluated using the IncuCyte live cell imager. No significant difference in cell growth was found. Data are representative of 3 independent experiments.

(D) Cells were injected into C57BL/6 mice, and tumors were measured by caliper every 2–3 days. Tumors were considered fully formed when they reached ~50mm³, at which point it was considered the time of tumor onset. Data were collected from a total of 18 mice per group from 2 independent experiments. *p < 0.05.

(E) Cells were injected into NSG mice and time-to-tumor onset was tracked, as in (D). Data were collected from a total of 5 mice per group.

(F) YUMM1.7 cells were engineered and injected as in (A). Tumors were considered fully formed when they reached ~50 mm³. Data were collected from a total of 6 mice per group from 2 independent experiments. *p < 0.05.

(G) Cells were injected into NSG mice and time-to-tumor onset was tracked, as in (F). Data were collected from a total of 5 mice per group.

See also Figures S2 and S3.