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. 2020 Jan 1;11(6):1299–1307. doi: 10.7150/jca.34423

Figure 2.

Figure 2

Inhibition of ROS reduces VK3-induced cell death in ovarian cancer cells. (A) Both cells were treated with VK3 (15 µM) for 8 or 16 h and ROS generation was determined using 50 µM DCFH-DA. DCF fluorescence intensity was detected by fluorescence microscopy (100×). (B) Quantification of DCF fluorescence intensity in (A). Data are presented as mean ± SD, n = 3. **P < 0.01 compared with control. (C) SKOV3 cells pretreated with 40 μM NAC for 1h were stained with Annexin V-FITC/PI. FACScan was used to count positively stained cells. (D) Quantitation of apoptotic rate in SKOV3 cells in (C). Data are presented as mean ± SD, n = 3. *P < 0.05 compared with 8 h VK3 treatment; #P < 0.05 compared with 16 h VK3 treatment. (E) The MTT assay was used to examine the cell viability with 40 µM NAC pretreatment followed by 15 µM VK3 culture. Data are presented as mean ± SD, n = 3. *P < 0.05 compared with VK3 treatment alone.