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. 2020 Jan 1;11(6):1299–1307. doi: 10.7150/jca.34423

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VK3 activates the Nrf2 pathway in SKOV3/DDP cells. (A) Both cells were treated as before. Nucleus extracts were subjected to immunoblot analysis with anti-Nrf2 and anti-LaminA/C. (B) Quantitation of nucleus Nrf2 protein level in (A). Data are presented as mean ± SD, n = 3. *P < 0.05 compared with untreated cells. (C) Total RNAs were prepared and NQO-1 and HO-1 mRNA levels were analyzed by RT-PCR. (D, E) Quantitation of HO-1 and NQO1 levels in (C). Data are presented as mean ± SD, n = 3. *P < 0.05 compared with SKOV3 cells. (F) The expression of HO-1 and NQO1 were examined by western blotting. (G, H) Quantitation of HO-1 and NQO1 levels in (E).Data are presented as mean ± SD, n = 3. *P < 0.05 compared with SKOV3 cells.