LGALS1 is upregulated or downregulated by LGALS1-Lt or LGALS1-shRNA in both SiHa and C33A cell lines. (A and B) Protein expression of LGALS1 in SiHa and C33A cells transfected with LGALS1-Lt was determined by Western blot analysis at 48 h post-transfection. (C and D) LGALS1 protein expression in SiHa and C33A cells transfected with four various LGALS1 shRNAs was detected by Western blot analysis 48 h post-transfection. Values are expressed as the mean ± SD. *P <0.05, **P <0.01. LGALS1, galectin-1; Control-Lt, control lentivirus; LGALS1-Lt, galectin-1 lentivirus; shRNA, small hairpin RNA.