Table 1.
Gene | Primer name | Primer sequence (5'-3') |
---|---|---|
LGALS1 | LGALS1 (XhoI). F | AATCTCGAGATGGCTTGTGGTCTGGTCGC |
LGALS1 (BamHI). R | TTGGATTCTCAGTCAAAGGCCACACATT | |
LGALS1-shA | LGALS1-shRNA-A | GATCCGGGTCGCCAGCAACCTGAATCTTCAAGAGAGATTCAGGTTGCGGCGACCTTTTTTG |
LGALS1-shB | LGALS1-shRNA-B | GATCCGACCTGTGCCTGCACTTCAACCTCAAGAGGGTTGAAGTGCAGGCACAGGTTTTTTTG |
LGALS1-shC | LGALS1-shRNA-C | GATCCGGCCAGATGGATACGAATTCAATCAAGAGTTGAATTCGTATCCATCTGGCTTTTTTG |
LGALS1-shD | LGALS1-shRNA-D | GATCCGGCCATCAACTACATGGCAGCTTCAAGAGAGCTGCCATGTAGTTGATGGCTTTTTTG |
Abbreviations: LGALS1, galectin-1; shRNA, small hairpin RNA.