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. 2020 Jan 14;11(6):1457–1467. doi: 10.7150/jca.35479

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p16 regulated IL1A expression in cervical cancer cell lines: (A) p16 transcript levels of Ca Ski sh-ctrl, Ca Ski sh-p16#1 and Ca Ski sh-p16#2 were determined by SYBR Green qRT-PCR analyses. (B) p16 expression levels of Ca Ski sh-ctrl, Ca Ski sh-p16#1 and Ca Ski sh-p16#2 were determined by Western blotting. GAPDH served as the loading control. (C) Cells proliferation assay of Ca Ski sh-ctrl, Ca Ski sh-p16#1 and Ca Ski sh-p16#2 for 60 hours. (D) A microarray identified 95 genes comparing Ca Ski sh-ctrl and Ca Ski sh-p16#1. The threshold was set as 1.5-fold changes and p < 0.05. (E) Heat maps of 8 genes involved in the regulation of cell proliferation. (F) IL1A transcript levels of Ca Ski sh-ctrl, Ca Ski sh-p16#1 and Ca Ski sh-p16#2 were determined by Taqman qRT-PCR analyses. (G) IL8 transcript levels of Ca Ski sh-ctrl, Ca Ski sh-p16#1 and Ca Ski sh-p16#2 were determined by SYBR Green qRT-PCR analyses.