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. 2020 Jan 14;11(6):1457–1467. doi: 10.7150/jca.35479

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p16 regulated IL1A expression in CDK6 dependent manner: CDK4 (A) and CDK6 (B) transcript levels of Ca Ski sh-ctrl+ctrl, Ca Ski sh-p16#1+ctrl, Ca Ski sh-p16#1+CDK4#1, Ca Ski sh-p16#1+CDK4#2, Ca Ski sh-p16#1+CDK6#1 and Ca Ski sh-p16#1+CDK6#2 were determined by SYBR Green qRT-PCR analyses and IL1A transcript levels (C) were determined by Taqman qRT-PCR analyses. CDK6 (D) and p16 (E) transcript levels of Ca Ski ctrl and Ca Ski CDK6R31C were determined by SYBR Green qRT-PCR analyses and IL1A transcript levels (F) were determined by Taqman qRT-PCR analyses. (G) Cell cycle (H) of synchronized Ca Ski sh-ctrl and Ca Ski sh-p16#1 was measured. (I) CDK6 and Cyclin D expression levels of synchronized Ca Ski sh-ctrl and Ca Ski sh-p16#1 were determined by Western blotting. GAPDH served as the loading control. (J) IL1A transcript levels of synchronized Ca Ski sh-ctrl and Ca Ski sh-p16#1 were determined by Taqman qRT-PCR analyses.