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. 2020 Jan 14;11(6):1457–1467. doi: 10.7150/jca.35479

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This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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HuR was involved in IL1A mRNA stability regulation: (A) A schematic diagram of IL1A 3'-UTR luciferase was constructed. (B) The activity of luciferase-based reporter of the IL1A 3'-UTR in Ca Ski cells was analyzed. (C) The interaction between RNA binding proteins and IL1A mRNAs was examined by RNA immunoprecipitation. Mouse IgG was included as a negative control. (D) HuR transcript levels of Ca Ski sh-ctrl, Ca Ski sh-HuR#1 and Ca Ski sh-HuR#2 were determined by SYBR Green qRT-PCR analyses. (E) The activity of luciferase-based reporter of the IL1A 3'-UTR in Ca Ski sh-ctrl, Ca Ski sh-HuR#1 and Ca Ski sh-HuR#2 was analyzed. (F) The stability of endogenous IL1A mRNA was affected by HuR knockdown. (G) The half-life of IL1A mRNA in Ca Ski sh-ctrl, Ca Ski sh-HuR#1 and Ca Ski sh-HuR#2 were calculated.