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. Author manuscript; available in PMC: 2020 Jun 19.
Published in final edited form as: Sci Transl Med. 2019 Jun 19;11(497):eaaw0790. doi: 10.1126/scitranslmed.aaw0790

Fig. 1. Patient-derived SSc fibroblasts maintain a fibrotic synthetic repertoire ex vivo.

Fig. 1.

(A) Heatmap of genes differentially expressed (rows) between healthy control and lesional SSc fibroblasts by RNA-seq (n = 3 biological replicates, FDR < 0.05). (B and C) mRNA and protein expression in control and SSc fibroblast lines. (D) mRNA expression in homogenized biopsies taken from diffuse SSc lesional skin (n = 5) versus healthy control skin (n = 4). (E) TGFB2 mRNA expression (green) measured by mRNA in situ hybridization of healthy control skin and lesional SSc skin sections (n = 4). Arrowheads indicate cells with high TGFB2 expression. Scale bars, 20 um. (F and G) mRNA expression upon stimulation of fibroblasts with exogenous TGFβ2 ligand. (H) TGFB ligand and TGFβ target gene expression upon siRNA knockdown of TGFB2 in primary fibroblasts. *P < 0.05; **P < 0.01; ***P < 0.001. ncontrol = 5 and nSSc = 6. Two-way Student t-test for single comparisons, or one-factor ANOVA with FDR correction for all experiments, except panel A and D-TGFB2 mRNA: Mann-Whitney. Black points represent individual biological replicates. Y-axes are varied across similar plots to aid visualization.