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. 2019 Nov 27;9(12):792. doi: 10.3390/biom9120792

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ELISA for the expression analysis of caspases and MAPK signaling pathway proteins. Over expressions of Caspase-3, 7, and 9 in Lanatoside C treated cells along with controls were observed. Upregulation of Phospho-P44/42 MAPK (Thr202/Tyr204), Phospho-p38 MAPK (Thr180/tyr182), MEK1, Phospho-MEK1 (Ser217/221), SAPK/JNK and Phospho-SAPK/JNK (Thr183/Tyr185) were assessed by ELISA in Lanatoside C treated MCF-7, A549, and HepG2 cells.