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Characterization of mAbs against tylosin and cross-reaction with tilmicosin.
| mAbs | Titer (103) | IC50 (ng/mL, Tylosin) |
IC50 (ng/mL, Tilmicosin) |
Cross-React with Tilmicosin (%) |
|---|---|---|---|---|
| L01 | 1.6 | 6.2 | 16.1 | 38.5 |
| L02 | 1.6 | 5.0 | 8.5 | 58.8 |
| L03 | 1.6 | 5.2 | 9.2 | 56.4 |
| L04 | 1.6 | 5.9 | 21.5 | 26.9 |
The mAbs were diluted 1:4000 in PBST (0.01 mol/L, pH 7.4) with 0.11 μg/mL of coating antigen.
