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. 2019 Dec 11;9(12):861. doi: 10.3390/biom9120861

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Recombinant Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase (PfHGXPRT) expression and purification. Isopropyl-β-D-1-thiogalactopyranoside (IPTG)-induced protein expression (A) and affinity purification (B) of PfHGXPRT was visualized by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) 12.5% (Upper panel) and confirmed by western blot (lower panel) using α-His horse-raddish-peroxidase (HRP)-conjugated antibodies (Sigma-Aldrich, St. Louis, MO, USA). Lane M represents a broad range molecular marker (Bio-Rad) in kilodaltons (kDa) shown on the left-hand side of the SDS-PAGE images. Lane: 2HR, 4HR, 6HR and O/N, respectively, represent cell lysates collected at 2, 4, 6 and 24 h post-IPTG induction. Lane E1–E7 represents the successfully purified PfHGXPRT protein elutions.