Inhibition plots of the ODC enzyme with multiple mutants of AZ95–228. The enzyme activity of ODC was inhibited by various multiple mutants of AZ95–228. The IC50 values of multiple mutants of AZ95–228 presented in Table 1 were derived by curve-fitting the inhibition plots. The molar ratio refers to AZ versus the ODC monomer. (A) AZ95–228_K153A/E164A/E165A (AZ95–228-3Mα1), (B) AZ95–228-3Mα1_D154A, (C) AZ95–228-3Mα1_E161A, (D) AZ95–228_K153A/D154A/E161A/E164A/E165A (AZ95–228-5Mα1), (E) AZ95–228-3Mα1_E142A, (F) AZ95–228-3Mα1_H171A, (G) AZ95–228-3Mα1_K178A, (H) AZ95–228_E142A/H171A/K178A, and (I) AZ95–228_E142A/K153A/D154A/E161A/E164A/E165A/H171A/K178A (AZ95–228-8M).