Table 1.

IC₅₀ values for AZ_95–228 and its mutants within the posterior part of AZ_95–228.

1 AZ Variants	Location	2 IC50 (μM)	3 Fold Change (IC50,mutant/IC50,WT)
AZ95–228	C-terminal domain	0.16 ± 0.01	1
AZ95–228_E142A	β5	0.31 ± 0.02	1.93
AZ95–228_K153A	α1	0.40 ± 0.10	2.5
AZ95–228_D154A	α1	0.27 ± 0.06	1.69
AZ95–228_E161A	α1	0.25 ± 0.06	1.56
AZ95–228_E164A	α1	0.30 ± 0.06	1.88
AZ95–228_E165A	α1	0.30 ± 0.07	1.88
AZ95–228_H171A	β6	0.27 ± 0.09	1.69
AZ95–228_K178A	Loop between β6 and α2	0.31 ± 0.05	1.94
AZ95–228-3Mα1	α1	4.13 ± 0.34	25.82
AZ95–228-3Mα1_D154A	α1	5.61 ± 0.35	35.08
AZ95–228-3Mα1_E161A	α1	6.31 ± 5.95	39.45
AZ95–228-5Mα1	α1	No Inhibition	-
AZ95–228-3Mα1_E142A	α1/β5	No Inhibition	-
AZ95–228-3Mα1_H171A	α1/β6	No Inhibition	-
AZ95–228-3Mα1_K178A	α1/loop_β6-α2	No Inhibition	-
AZ95–228_E142A/H171A/K178A	β5/β6/loop_β6-α2	No Inhibition	-
AZ95–228-8M	β5/α1/β6/loop_β6-α2	No Inhibition	-

¹ AZ_95–228-3Mα1: AZ_95–228_K153A/E164A/E165A, AZ_95–228-5Mα1: AZ_95–228_K153A/D154A/E161A/E164A/E165A, AZ_95–228-8M: AZ_95–228_E142A/K153A/D154A/E161A/E164A/E165A/H171A/K178A. ² All IC₅₀ values were derived from fitting the inhibition curves of ODC shown in Figure 2 and Figure S2. ³ Fold change was the ratio of IC₅₀ of the mutant versus IC₅₀ of WT.