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. 2019 Nov 21;9(12):759. doi: 10.3390/biom9120759

Figure 1.

Figure 1

The effects of Zhankuic acids A, B and C (ZA-A, ZA-B and ZA-C) on human P-glycoprotein (P-gp). (a) Intracellular calcein fluorescence with or without ZA-A, ZA-B or ZA-C pretreatment in ABCB1/Flp-InTM-293 cell line (over-expressing human P-gp). Verapamil 5 μM was used as a positive control. * denotes p < 0.05 compared with the intracellular calcein fluorescence in control group. (b) The dose-dependent effects of ZA-A, ZA-B or ZA-C on calcein retention in ABCB1/Flp-InTM-293. * denotes p < 0.05 compared with the intracellular calcein fluorescence in control group. (c), (d) There were no significant ABCB1 mRNA expression differences after treating the ABCB1/Flp-InTM-293 or KB/VIN with 10 μM ZA-A, ZA-B or ZA-C for 72 h. © The conformation of P-gp was not influenced under the treatment of 10 μM ZA-A, ZA-B or ZA-C. Vinblastine was used as a positive control.