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. 2019 Nov 21;9(12):759. doi: 10.3390/biom9120759

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The cell cycle and apoptosis analyses of ZA-A and paclitaxel combinatorial treatment. (a), (b), (c) and (d) The cell cycle distribution of 72 h treatment in Flp-In^TM-293, ABCB1/Flp-In^TM-293, HeLaS3 and KB/VIN, respectively. Data were presented as mean ± SE of at least three experiments, each in triplicate. (e) The apoptosis phenomenon of 72 h treatment in KB/VIN cell line. Apoptosis and necrosis status of each sample was determined by annexin V (X-axis FITC) and PI (Y-axis PI). Cells distributed in Q1, Q2, Q3 and Q4 represent necrosis, late-apoptosis and normal and early-apoptosis, respectively.

The cell cycle and apoptosis analyses of ZA-A and paclitaxel combinatorial treatment. (a), (b), (c) and (d) The cell cycle distribution of 72 h treatment in Flp-In^TM-293, ABCB1/Flp-In^TM-293, HeLaS3 and KB/VIN, respectively. Data were presented as mean ± SE of at least three experiments, each in triplicate. (e) The apoptosis phenomenon of 72 h treatment in KB/VIN cell line. Apoptosis and necrosis status of each sample was determined by annexin V (X-axis FITC) and PI (Y-axis PI). Cells distributed in Q1, Q2, Q3 and Q4 represent necrosis, late-apoptosis and normal and early-apoptosis, respectively.