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. 2019 Dec 1;9(12):810. doi: 10.3390/biom9120810

Figure 2.

Figure 2

Effects of LSD1 knock-out on HCC cell viability in vitro. LSD1 knock-out SNU-423 cells and SNU-475cells were generated by the CRISPR/Cas9 system with two guide RNAs and were obtained by treatment with Dox. (A) Western blot analysis of LSD1, H3K4me2, H3K4me1, H3K9me2, H3K9me1, histone H3, and α-Tubulin in SNU-423-KO and SNU-475-KO cells. (B) Colony formation assays were conducted to determine the proliferation of infected HCC cells. All data are represented as mean ± s.d. * p < 0.05. (C) Cell proliferation assays were performed to determine the cell viability of infected HCC cells with the CCK-8 kit. All data are represented as mean ± s.d. ** p < 0.01; *** p < 0.001.