Figure 3.

ClpG has higher disaggregation activity towards complex aggregates. (a) Soluble ³H-labeled proteins from an E. coli ΔclpB cell lysate (including Luciferase) were heat shocked to 46 °C for 15 min. Protein aggregates were isolated by centrifugation and resuspended in buffer. Disaggregase activities were monitored by determining the amount of solubilized ³H-labeled proteins (b,c) or Luciferase refolding (d,e). Disaggregation reactions with ClpB and ClpB-K476C included the cooperating DnaK system. Luciferase activities before heat shock were set to 100%. Rates of protein solubilization (c) and Luciferase reactivation (e) were determined. Standard deviations are given (n = 3).