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. 2019 Dec 13;9(12):874. doi: 10.3390/biom9120874

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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qRT-PCR analysis of genes related to carotenoid metabolic pathways after silencing. Plastids are the sites for the biosynthesis and accumulation of plant carotenoids. PAP, plastid lipid-associated protein; PSY, phytoene synthase; PDS, phytoene desaturase; ZDS, ζ-carotene desaturase; ZISO, 15-cis-ζ-carotene isomerase; CRTISO, carotene isomerase; LCYE, lycopene ε-cyclase; LCYB, lycopene β-cyclase; CYC-B, chromoplast-specific lycopene β-cyclase; BCH, β-carotene hydroxylase; ECH, ε-carotene hydroxylase; ZEP, zeaxanthin epoxidase; VDE, violaxanthin de-epoxidase; NXY, neoxanthin synthase; CCD, carotenoid cleavage dioxygenases. Different letters indicate significant differences between negative control and silenced fruits (One-way ANOVA, p < 0.05).