Figure 2.

Acetone extracts of Pseudocyphellaria granulata and physciosporin inhibit colorectal cancer (CRC) cell stemness. (A) Representative images of spheroid formation of DLD1, Caco2, and HT29 cells treated with P. granulata crude extract or physciosporin for 14 days. (B–D) Quantitative analysis of relative spheroid number in DLD1 (B), Caco2 (C), and HT29 (D) cultures. Quantitative data were obtained from three independent experiments, n = 3. Data are means ± standard error of the mean (SEM), and statistical analysis was performed by one-way ANOVA. *** p < 0.001 vs. DMSO-treated CRC cells.