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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Dev Biol. 2019 Nov 14;458(2):164–176. doi: 10.1016/j.ydbio.2019.11.007

Fig. 2: Titration of 24 hour doxycycline treatment for Adamts18en-rtTA tet-on induction demonstrates consistency in activation through two rtTA reporter mice.

Fig. 2:

For all kidneys collected, various concentrations of doxycycline (indicted in figure) in 5% sucrose water was given to the pregnant mouse 24hr prior to dissection at E15.5. A-D: AE-rtTA;TRE-H2BGFP kidneys show GFP expression in the UB tip population starting at 0.1mg/ml. E-H: AE-rtTA;TRE-GFP/LacZ kidneys have very weak GFP expression from 0.1mg/ml to 2mg/ml. I-L: LacZ staining shows the presence of β-galactosidase in AE-rtTA;TRE-GFP/LacZ kidneys within the ureteric bud tip population that matches the GFP expression in F-H. Line = 200μm.