Skip to main content
. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Dev Biol. 2019 Nov 14;458(2):164–176. doi: 10.1016/j.ydbio.2019.11.007

Fig. 3: Embryonic mouse tissue of AE-rtTA;TRE-H2BGFP highlights the temporal and spatial expression of Adamts18en-rtTA.

Fig. 3:

For all embryos collected (except K-M), 2mg/ml doxycycline in 5% sucrose water was given to the pregnant mouse 24hr prior to dissection. A: No GFP expression observed within the E8.5 embryo. B: GFP expression is within the nephric duct at E9.5. C: At E10.5, GFP expression is present in the nephric duct and limb buds. D-F: Kidneys at E11.5 (D), E13.5 (E), and E15.5 (F) with GFP expression restricted to the distal ends of the ureteric epithelium. G-J: Sectioned E14.5 embryo stained with Hoechst 33342 and demonstrating native GFP expression pattern within the whole embryo (G), ureteric epithelium of the kidney (H), epidermis (I), and digits (J). K-M: Volume projections of ureteric bud tips from a whole-mount stained E16.5 AE-rtTA;TRE-H2BGFP;Wnt11RFP kidney that was administered 2mg/ml doxycycline 48hr before collection. Tissue was stained with RFP, GFP, and Troma (epithelial marker) antibodies. Line in A-H, J = 200μm; line in I, K-M = 20μm.