Table 4.
Descriptions of Lacipirellula gen. nov. and Lacipirellula parvula sp. nov.
| Genus name | Lacipirellula | |
| Species name | Lacipirellula parvula | |
| Genus status | gen. nov | |
| Genus etymology | La.ci.pi.rel’lu.la. L. masc. n. lacus lake; N.L. fem. n. Pirellula a bacterial genus; N.L. fem. n. Lacipirellula a lake-dwelling Pirellula | |
| Type species of the genus | Lacipirellula parvula | |
| Specific epithet | – | parvula |
| Species status | – | sp. nov |
| Species etymology | – | par'vu.la. L. fem. adj. parvula small |
| Description of the new taxon and diagnostic traits | Gram-stain negative, ellipsoidal cells that occur singly or in aggregates. Reproduce by budding; daughter cells are motile by means of a polar flagellum. Mature cells are non-motile. Free cell poles are covered with crateriform pits. Cells occurred singly, in pairs, in rosettes composed of 3 cells or in aggregates. Stalks are not formed. Non-pigmented. Oxidase- and catalase-positive. Mesophilic. Chemoorganotrophic. Sugars are the preferred growth substrates. Capable of hydrolyzing several polysaccharides but not cellulose or chitin. Micro-aerobic and facultatively anaerobic. Anaerobic growth occurs by means of fermentation. The major fatty acids are C16:0, C18:1ɷ9c, and C16:1ɷ7c. The major polar lipid is dimethylphosphatidylethanolamine. The genus belongs to the family Lacipirellulaceae of the order Pirellulales. | Colonies are small (0.5–1 mm in diameter), circular and unpigmented. The cells are 0.5-0.9 μm wide and 0.9–1.4 μm long. The temperature range for growth is 10–30 °C with the optimum at 20–25 °C. The pH range for growth is 5.0–7.5 with the optimum at 6.5. Growth does not occur at NaCl concentrations above 0.5%. Do not require sea salt (ASW). Facultatively anaerobic, capable of microaerobic growth. Growth under oxic and micro-oxic conditions occurs on glucose, fructose, galactose, lactose, cellobiose, maltose, mannose, melibiose, melezitose, rhamnose, ribose, trehalose, sucrose, xylose, raffinose, N-acetylglucosamine, pyruvate, salicin, arbutin, glycerol, tween 80, glucuronic acid, lactate, pyruvate, glycerin, sorbitol and dulcitol. Capable of hydrolyzing aesculin, arabinogalactan, chondroitin sulfate, dextrin, laminarin, locust bean gum, gelatin, starch, xanthan gum, pectin and xylan. Cannot utilize fructose, raffinose, sorbose, ribose, methanol, acetate, benzoate, caproate, citrate, fumarate, glutarate, malate, propionate, mannitol, tartrate, alanine, arginine, asparagine, aspartate, cysteine, cystine, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, norleucine, ornithine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine or valine. Cannot hydrolyze casein, chitosan, chitin, starch, carboxymethylcellulose and cellulose. Grow anaerobically by fermentation of glucose, lactose, trehalose, sucrose, and xanthan gum. The end product of glucose fermentation is lactate. Urease is negative. Dissimilatory nitrate reduction is positive (API 20NE strip). In the API ZYM strip, alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β-galactosidase are present but trypsin, α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminidase, α-galactosidase, α-fucosidase, lipase (C4), cystine arylamidase, α-chymotrypsin, β-glucuronidase and α-mannosidase are absent. The major fatty acids are C18:1ω9c, C16:0 and 16:1ω7c. The major polar lipid is dimethylphosphatidylethanolamine. Resistant to ampicillin, carbenicillin, chloramphenicol, gentamicin, neomycin, kanamycin, streptomycin, but sensitive to rifampicin and tetracycline. |
| Country of origin | – | Russian Federation |
| Region of origin | – | Vologda region |
| Date of isolation | – | 25/10/2017 |
| Source of isolation | – | Boreal lake water |
| Sampling date | – | 08/2017 |
| Latitude | – | 58°43′28.5ʺN |
| Longitude | – | 37°39′07ʺE |
| Genome accession number | – | GenBank: AP021861 |
| Genome status | – | complete |
| Genome size | – | 6.92 |
| GC mol% | – | 61.17 |
| Number of strains in study | – | 1 |
| Information related to the Nagoya Protocol | – | Not applicable |
| Designation of the Type Strain | – | PX69T |
| Strain Collection Numbers | – | KCTC 72398T = CECT 9826T = VKM B-3335T |