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. 2020 Jan 28;30(4):1013–1026.e7. doi: 10.1016/j.celrep.2019.12.023

Figure 3.

Figure 3

Chronic Infection Triggers a Sustained GC Response with Prolonged Plasma Cell and Memory B Cell Output

(A) We infected AIDrep mice with rARM or rCl13 on day 0, followed by TAM administration on day 0 and day 5 (early), day 10 and day 15 (intermediate), or day 30 and day 35 (late), respectively.

(B) EYFP-expressing cells were analyzed 2 months after infection. Representative FACS plots are gated on EYFP+ cells (see Figure S3). Numbers in FACS plots indicate percentages of gated cells among EYFP+ cells.

(C–E) EYFP-expressing GC B cells (C), MemB cells (D), and ASCs (E) in the spleen were enumerated. Dotted lines indicate background levels of EYFP-expressing cells in uninfected control mice.

(F) Representative histological spleen sections from rCl13- and ARM-infected mice in the early labeled group.

Magnification bar: 50 μm. Bars represent means ± SEM, n = 4–5 (C–E) and n = 6 from three datasets (F). N = 2. Two-way ANOVA with Bonferroni’s post-test for multiple comparisons. ∗p < 0.05, ∗∗p < 0.01. See also Figure S3.