Figure 2.

Protein Turnover Rates in CR and GF Mice

(A–F) Histograms of protein half-lives along the gastrointestinal tract divided into 50 bins. The median half-life and the number of protein half-lives for each of the locations are stated on top of the corresponding histogram. DE, duodenum epithelial cells; MJE, middle jejunum epithelial cells; IE, ileum epithelial cells; PCE, proximal colon epithelial cells; DCE, distal colon epithelial cells; IM, ileum mucus; DCM, distal colon mucus as explained in Figure 1A. See also Figure S3.

(G) Boxplots represent the distribution of turnover rates for all of the proteins, 25th and 75th percentiles, and the whiskers indicate the values within 1.5 times the interquartile range [IQR]. The dotted line represents the median of protein turnover rates.

(H and I) Protein abundances and turnover rates for lysine transporters Slc3a1 and Slc6a14. Dots connected with solid line represent the specific protein turnover rate; error bars represent coefficient of variance (CV). Dotted lines represent median protein turnover rate of all proteins. Bars represent median protein abundance with standard deviation (SD). Asterisk above turnover rate dots represents protein turnover rate difference GF versus CR, with p < 0.1 based on Perseus (version 1.5.0.0) Significance A test. Asterisk above bars represents protein abundance difference GF versus CR, with p < 0.05 based on Significance A test.

(J and K) Median protein turnover rate (dotted line) and turnover rates of proteins used for cell turnover estimation in CR (J) and GF mice (K). Tfam - Turnover rate of Tfam. Hist - turnover rates of histones (Hist1h1b, Hist1h1c, Hist1h1d, Hist2h2aa1, and Hist1h4a).

(L) Median protein turnover rates and estimated cell turnover rate for CR and GF mice.