Figure 5.

Turnover Rate and Protein Abundance for Mucus Components

(A) Turnover and abundance of Muc2, Clca1, Fcgbp, and Zg16. Bars represent protein abundance calculated based on MS peak intensity with SD; dots with connecting lines represent specific protein turnover rate and error bars coefficient of variance; dotted lines represent median protein turnover rate of all proteins; asterisk above dot represents protein turnover rate difference GF versus CR, with p < 0.1 based on Significance A test. GF, blue; CR, red.

(B) Turnover rate of mucus main components and estimated cell turnover (median of Tfam, Hist1h1b, Hist1h1c, Hist1h1d, Hist2h2aa1, and Hist1h4a) on the background of all of the turnover rates.

(C) Turnover of all proteins: black empty boxes represent all proteins in epithelial cells; green filled boxes represent all of mucus proteins; and empty green boxes represent all secreted proteins in mucus (mucus proteins with a signal sequence). Boxes represent the medians and 25th and 75th percentiles, and the whiskers indicate the values within 1.5 times the IQR. Median protein turnover rate for mucus and for secreted proteins in mucus has been compared to epithelial cells; ns, non-significant. ^∗∗∗p ≤ 0.0002; ^∗∗∗∗p ≤ 0.0001 as determined with one-way ANOVA followed by Dunnett’s multiple comparison test.

(D) Protein turnover of mucus components in ileum and distal colon for epithelial cells (filled dots) and mucus (empty dots) in GF (blue) and CR (red) mice.