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. 2020 Jan 28;13:813–825. doi: 10.2147/OTT.S237127

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© 2020 Gao et al.

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NUSAP1 downregulation induced cell cycle arrest and apoptosis in BLCA cells. (A). GSEA suggests that NUSAP1 is closely related to the G2/M cell cycle phase. (B and C). Cell cycle analysis via flow cytometry. **P<0.01 vs NC. (D). Western blotting analysis of CDK1 and cyclin B1 protein expression. (E and F). Apoptosis was analyzed by flow cytometry. **P<0.01 vs NC (5637), *P<0.05 vs NC (T24). (G). Western blotting analysis detected the protein expression levels of Bax, Bcl-2 and cleaved-caspase3.