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. 2020 Jan 28;13:813–825. doi: 10.2147/OTT.S237127

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© 2020 Gao et al.

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NUSAP1 downregulation in BLCA cells enhanced GEM chemosensitivity and suppressed cell migration, invasion, and EMT. (A). The chemosensitivity of BLCA cells to GEM was determined by a Cell Counting Kit-8 assay. *P<0.05, **P<0.01, ***P<0.001 vs NC (5637) or NC (T24). (B and C). A wound healing assay was performed to detect the migration of cells (100× magnification). **P<0.01, vs NC (5637) or NC (T24). (D and E). Transwell assays were performed to evaluate the migration and invasion of cells (100× magnification). **P<0.01, vs NC (5637) or NC (T24). (F). Western blotting analysis detected the protein expression levels of E-cadherin, vimentin and N-cadherin.