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. 2020 Jan 28;13:813–825. doi: 10.2147/OTT.S237127

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© 2020 Gao et al.

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NUSAP1 regulated the epithelial-mesenchymal transition of BLCA via the TGF-β signaling pathway. (A). GSEA revealed that NUSAP1 expression was positively correlated with the TGFBR complex and the Smad2/3 pathway in BLCA. (B). Gene correlation analysis of NUSAP1 and TGFBR1 obtained from Gene Expression Profiling Interactive Analysis (GEPIA, http://gepia.cancer-pku.cn/). ***P<0.001. (C). Real-time PCR was performed to detect the relationship between NUSAP1 and TGFBR1. **P<0.01, vs NC (5637) or NC (T24). (D). Western blotting analysis of the protein expression levels of TGFBR1, Smad2/3, p- Smad2/3, E-cadherin, and vimentin. (E and F). Wound healing assays were performed to detect the migration of cells (100× magnification) *P<0.05, **P<0.01. (G and H). Transwell assays were performed to evaluate the migration and invasion of cells (100× magnification). *P<0.05, **P<0.01, ***P<0.001. (I). Western blotting analysis of the protein expression levels of Smad2/3, p- Smad2/3, E-cadherin, and vimentin.