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. 2020 Jan 29;14:417–428. doi: 10.2147/DDDT.S228047

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© 2020 Zhang et al.

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Baicalin increases UVB-induced autophagy in HSFs. HSFs were pretreated with Bafilomycin A1 (100 nM) for 2 h, then treated with 25 ng/mL baicalin. (A) Representative confocal images of GFP-LC3 in fibroblasts. Scale bars = 50μm. (B) The percentage of cells with induced autophagy was determined as the percentage of GFP-LC3 cells with greater than 10 GFP-LC3 puncta per cell (means ±SEM of the independent experiments, *p < 0.05). (C) LC3-II and Beclin1 expressions were detected by Western blotting analysis. GAPDH was used as a loading control. The concentration of baicalin in these studies was 25 ng/mL.