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. 2020 Jan 29;14:417–428. doi: 10.2147/DDDT.S228047

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© 2020 Zhang et al.

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3-MA inhibits the effects of Baicalin on UVB-induced autophagy and apoptosis in HSFs. (A) Representative confocal images of GFP-LC3 in fibroblasts. Scale bars = 50μm. (B) The percentage of cells with induced autophagy was determined as the percentage of GFP-LC3 cells with greater than 10 GFP-LC3 puncta per cell (means ±SEM of the independent experiments, *p < 0.05 versus UVB, #p < 0.05 versus UVB+Baicalin). (C) LC3-II and Beclin1 expressions were detected by Western blotting analysis. GAPDH was used as a loading control. (D) C-caspase3 expression was detected by Western blotting analysis. GAPDH was used as a loading control. (E, F) Cellular apoptosis was assayed by annexin V-FITC and PI counterstaining, and analyzed with flow cytometry. The original flow cytometry figures are shown in (E) and the apoptosis rates are shown in (F). Values are given as means±SEM (n=5). *p < 0.05 versus UVB, #p < 0.05 versus UVB+Baicalin. The concentration of baicalin in these studies was 25 ng/mL.