Fig. 3.

Localization of CSF1 in the immune tissues. Tissue sections were processed as in Materials and Methods. (A and B) CSF1 staining of spleen from a 16 week old chicken. CSF1 staining is localised to the ellipsoid surrounding the penicillar capillary. CSF1 (Red), and DAPI (blue), scale bar = 20 μm. (C–F) CSF1 staining on bursa of Fabricius from a 3 day old chick. CSF1 staining is localised to the cells at the cortico-medullary junction. CSF1 (Red), IgY (green) and DAPI (blue). (C and E) Objective lens 20 × ; scale bar = 20 μm. (D and F) Objective lens 40 × ; scale bar = 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)