Fig. 4.

Flow cytometric analysis of surface expression of CSF1R on BMDM. BMDMs were cultured with CSF1 for 6 days. At this point, half of the cells were starved of CSF1 overnight (no CSF1) and the other half were cultured with CSF1 (with CSF1). Following a further 24 h of culture, BMDM were stimulated with LPS for 3 h. Cells were stained with either anti-CSF1-AF647 or CSF1-Fc-AF647. All histograms are representatives of three independent experiments. (A) Comparison of the staining profiles of CSF1 and CSF1-Fc between cells starved of CSF1 (dotted line) or cultured with CSF1 (solid line). (B) Comparison of the staining profiles between cells non-stimulated (solid line) or stimulated with LPS (dotted line) for 3 h. Cells were cultured with CSF1 or starved of CSF1 as in A. ▲ Isotype controls.