Fig. 5.

HPG can label Intracellular Rickettsiales species.

The methionine probe HPG was added to infected cells for 30 min prior to fixation. HPG was reacted with an azide-conjugated fluorophore to reveal cells actively undergoing protein synthesis (green). Host cell nuclei are shown in blue (DAPI). Scale bar = 5 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)