Table 1.

Effects of diet, mucin type, gut segment and crypt-villus fragment on mucin staining intensity in the broiler chickens

Factor	d.f.f	Chi-square	P*
Crypts
Dieta	3	3.751	0.290
Mucin typeb	2	22.566	< 0.001
Gut segmentc	3	63.140	< 0.001
Fragmentd	2	247.461	< 0.001
Villi
Diet	3	25.497	< 0.001
Mucin type	2	4.510	0.100
Gut segmente	2	571.512	< 0.001
Fragment	2	1.488	0.475

^aFour dietary treatments: C = control; HI5 = 5% inclusion level of Hermetia illucens; HI10 = 10% inclusion level of Hermetia illucens; HI15 = 15% inclusion level of Hermetia illucens

^bThree types: neutral, acidic sialylated and acidic sulfated mucins

^cFour gut segments: duodenum, jejunum, ileum and caecum

^dThree fragments: base, midsection and tip

^eThree gut segments: duodenum, jejunum and ileum

^fDegrees of freedom

^*Statistical significance: P < 0.05. Statistical trend: P ≤ 0.10