. 2020 Feb 3;11:11. doi: 10.1186/s40104-019-0413-y

Table 2.

Least square means of mucin staining intensity in the intestinal crypts of the broiler chickens in relation to diet, mucin type, gut segment and crypt fragment

	Factor	Factor levels	Mucin staining intensity^1,2
Crypts	Diet	C	1.23 ± 0.03
		HI5	1.27 ± 0.03
		HI10	1.27 ± 0.03
		HI15	1.30 ± 0.03
	Mucin type	Neutral	1.32 ± 0.03^A
		Acidic sialylated	1.31 ± 0.02^A
		Acidic sulfated	1.18 ± 0.02^B
	Gut segment	Duodenum	1.26 ± 0.03^B
		Jejunum	1.20 ± 0.03^BC
		Ileum	1.45 ± 0.03^A
		Caecum	1.19 ± 0.03^C
	Fragment	Base	1.59 ± 0.03^A
		Midsection	1.17 ± 0.02^B
		Tip	1.10 ± 0.02^C
Villi	Diet	C	1.90 ± 0.04^A
		HI5	1.87 ± 0.04^A
		HI10	1.72 ± 0.04^B
		HI15	1.66 ± 0.04^B
	Mucin type	Neutral	1.79 ± 0.04^ab
		Acidic sialylated	1.84 ± 0.04^a
		Acidic sulfated	1.74 ± 0.03^b
	Gut segment	Duodenum	1.23 ± 0.03^C
		Jejunum	1.95 ± 0.04^B
		Ileum	2.37 ± 0.04^A
	Fragment	Base	1.75 ± 0.03
		Midsection	1.81 ± 0.04
		Tip	1.80 ± 0.04

C control, HI5 5% inclusion level of Hermetia illucens, HI10 10% inclusion level of Hermetia illucens, HI15 15% inclusion level of Hermetia illucens

¹Data are represented as mean of counts ± SEM

²Means with different superscript letters (a, b or A, B, C) within the same column per predictor (i.e. diet, mucin type, gut segment or fragment) differ significantly (P < 0.05 or P < 0.01, respectively)