Fig. 2.

Engineering of CGMMV as a VIGS vector with different size CP subgenomic promoters. a Schematic representation of pV61, pV92, pV112 and pV190. pV- is a pXT1-CGMMV derivative that contains a direct repeat of the 61-, 92-, 112- and 190-bp putative CGMMV CP subgenomic promoter and a restriction enzyme site (BamHI) between CP subgenomic promoters. b pV190 caused mild systemic symptoms on cucurbits and N. benthamiana. Bar = 1, 3, 3, 3 and 1 cm, respectively.c RT-PCR detection of viral RNA showing that pXT1-CGMMV and pV190 are infectious in cucurbits and N. benthamiana. M, Marker2000; WT, wild type (pXT1-CGMMV); CK, negative control; W, M, C, G and Nb indicate watermelon, melon, cucumber, bottle gourd and N. benthamiana, respectively