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. 2020 Jan 29;13:903–914. doi: 10.2147/OTT.S230847

Figure 5.

Figure 5

GSEA enrichment plots of focal adhesion and metastasis pathways. (A, D) Genes in the focal adhesion and metastasis pathways showed significant enrichment in ST6GAL2 high versus ST6GAL2 low in breast cancer. The top portion of the figure plots the enrichment scores (ES) for each gene, whereas the bottom portion of the plot shows the value of the ranking metric moving down the list of ranked genes. MCF-7 and T47D cells were transduced with lentivirus to knockdown ST6GAL2 or a negative control (NC). (B, C) Real-time PCR and Western blot analysis identified significant decrease in ICAM-1, VCAM-1, and CD24 expression in MCF-7 and T47D cells; (E, F) real-time PCR and Western blot analysis identified significant decrease in MMP2, MMP9, and CXCR4 expression in MCF-7 and T47D cells. Results are reported as mean ± SD (n=3) and statistically analyzed with two-way ANOVA followed by post-hoc Tukey’s test. *P<0.05, **P<0.01 compared with NC.