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. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: Neurobiol Dis. 2019 May 17;129:118–129. doi: 10.1016/j.nbd.2019.05.009

Fig. 1. Pridopidine protects striatal neurons from mutant Huntingtin induced toxicity.

Fig. 1.

(A) Representative images of GFP transfected striatal neurons. Cells were transfected as stated, treated for 48 h, fixed using 4% paraformaldehyde in PBS, and stained with Hoechst. Healthy non-condensed nuclei (arrow) and apoptotic condensed nuclei (arrowhead) are indicated. Scale bar: 25 μm.

(B) Quantification of nuclear condensation assay in striatal neurons. CD1 primary striatal neurons transfected at DIV5 were treated with 1 μM pridopidine or 20 ng/mL BDNF in the culture media for 48 h before nuclei were stained with Hoechst. Quantification of nuclear staining intensity in transfected cells was performed using Volocity. Results presented as individual values plus means ± S.E. of the percentage of dead cells. *** p < .001 vs DMSO, ANOVA with Bonferroni post-hoc test. (n = 6 independent neuronal preparations).