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. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: Neurobiol Dis. 2019 May 17;129:118–129. doi: 10.1016/j.nbd.2019.05.009

Fig. 2. In nuclear condensation assays, pridopidine protects cortical neurons from mutant Huntingtin toxicity.

Fig. 2.

(A) Quantification of 3PPP treated cortical neuron cell death. Transfected CD1 primary cortical neurons were treated with a given 3PPP concentration in the culture media for 48 h before nuclei were stained with Hoechst. Quantification of nuclear staining intensity in transfected cells was performed using Volocity. Results presented as individual values plus means ± S.E. of the percentage of dead cells. ** p < .01 vs Htt-82Q untreated cells, *** p < .001 vs Htt-82Q untreated cells, ANOVA with Bonferroni post-hoc test. (n = 8 independent neuronal preparations).

(B) Quantification of pridopidine treated cortical neuron cell death. Transfected CD1 primary cortical neurons were treated with a given pridopidine concentration in the culture media for 48 h before nuclei were stained with Hoechst. Quantification of nuclear staining intensity in transfected cells was performed using Volocity. Results presented as individual values plus means ± S.E. of the percentage of dead cells. *** p < .001 vs Htt-82Q untreated cells, ANOVA with Bonferroni post-hoc test. (n = 8 independent neuronal preparations).