FIG 5.

HBO1 is essential for H3K14ac. (A to D) 293T, MCF7, and HeLa parental cells were treated with Dox for 3 days and assessed for histone acetylation levels 1 week (A to C) or 4 weeks (D) after Dox treatment. (E) HeLa parental cell acetylation levels were quantified by densitometry. Ponceau S staining was conducted as a loading control for the respective Western blots (see Fig. S4 in the supplemental material). Each lane represents one parental cell line transduced with HBO1 sgRNA1 or sgRNA2. HBO1 protein levels in the respective parental cell lines are shown in Fig. 1A. (F to K) 293T, MCF7, and HeLa clonal cell lines were assessed for histone acetylation levels approximately 4 to 6 weeks after the initial Dox treatment. Histone acetylation levels were quantified by densitometry in panels G, I, and K. Ponceau S staining was conducted as a loading control for the respective Western blots (Fig. S4). Each lane represents one clonal cell line transduced with HBO1 sgRNA1 or sgRNA2. HBO1 protein levels in the respective clonal cell lines are shown in Fig. 1B. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Data are presented as means ± standard deviations from 2 parental cell lines, each transduced with HBO1 sgRNA1 or -2, in panel E. Data are presented as means ± standard errors of the means from 4 clonal cell lines, i.e., 2 each transduced with HBO1 sgRNA1 or sgRNA2, in panels G, I, and K.