FIG 6.

HBO1 siRNA-mediated knockdown reduces H3K14ac levels. Untreated HeLa cells, HeLa cells treated with Lipofectamine alone, or HeLa cells with Lipofectamine-mediated transfection of nonsilencing RNA, HBO1 siRNA^Miotto, and HBO1 siRNA^s253 were assayed for histone lysine acetylation. Transfection of HBO1 siRNA^Miotto and HBO1 siRNA^s253 resulted in reduced levels of H3K14ac but no changes in H3K9ac or H4K5ac, H4K8ac, H4K12ac, or H4K16ac. Western blots were performed 72 h after treatment. Western blots for the 24-h and 48-h time points are displayed in Fig. S5 in the supplemental material.