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. 2020 Jan 29;14:395–405. doi: 10.2147/DDDT.S222694

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© 2020 Wei et al.

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Effect of Genipin on cell autophagy in OSCC. SCC-25 and SCC-9 cells were treated with three concentrations of Genipin (10, 30, and 60 μM). (A) The autophagy relevant protein (P62, Beclin1, LC3II/LC3I) levels in SCC-25 were detected by Western blotting. (B) The autophagy relevant protein (P62, Beclin1, LC3II/LC3I) levels in SCC-9 were detected by Western blotting. (C) LC3 (LC3II)⁺ puncta in SCC-25 and SCC-9 cells were measured by immunofluorescence assay, respectively. (D) After added the autophagy inhibitor 3-MA, LC3 (LC3II)⁺ puncta in SCC-25 and SCC-9 cells were measured by immunofluorescence assay, respectively. *p<0.05, **p<0.01. Data are presented as mean ± SD, and all the experimental in triplicate.