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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Int J Biochem Cell Biol. 2019 Sep 19;116:105616. doi: 10.1016/j.biocel.2019.105616

Fig. 5.

Fig. 5.

Citrate synthase is increased in TH mice liver and kidney. A) The expression of various crucial metabolic enzymes involved in the citric acid cycle and gluconeogenesis, including citrate synthase (CS), pyruvate dehydrogenase (PDH), pyruvate dehydrogenase kinase 1 (PDHK1), and mitochondrial phosphoenolpyruvate carboxykinase (mtPEPCK), was assessed in TH and B6 mice liver and kidney (B) by Western blot analyses. The quantitative analyses of metabolic enzymes by densitometry were normalized to the expression of GAPDH and Ponceau S stained membranes (bottom panels) as equal loading controls. Results are expressed as the mean ± SD of at least three experiments for each mouse strain and presented as a percentage of B6 mice. Unpaired Student’s t-tests (2-tailed) were performed to for statistical analyses and no significant difference was observed between TH and B6 mice.