Figure 3.

Canonical transient receptor potential‐6 (TRPC6) inactivation did not affect course of renal pathology. A, Typical examples of PAS‐stained glomeruli from streptozotocin (STZ)‐ or vehicle‐treated Trpc6 ^wt/wt and Trpc6 ^del/del rats as indicated. B, Semi‐quantitative analysis of mesangial expansion in glomeruli carried out by an observer blind to treatment group or genotype. Two‐way ANOVA indicates increase in mesangial expansion in STZ‐treated animals with no interaction between drug treatment and genotype. There is a marked trend toward increased mesangial expansion in Trpc6 ^del/del rats. C, Immunoblot analysis of α‐SMA revealed no effect of either STZ treatment or genotype, suggesting that fibrosis in these animals was minimal