Figure 9.

Effect of IFNγ and CD40L (Comb) gene‐transfection therapy on systemic immune responses. Spleen cells were collected from the LM8 tumor‐bearing mice with the indicated treatments at 7 d after the second treatment. Cytotoxic activity of the cells against LM8 (A), BW5147 (B), and YAC‐1 (C) was evaluated in vitro. The experiments were repeated twice. Each experiment was performed using three mice in each treatment group. A typical result is shown. All mice were humanely euthanized according to the criteria in Section 2. Results are expressed as mean ± SE. *P < .05, **P < .01 vs the [Untreated] group, ^† P < .05 vs the [Comb (IT) + DC] and [Comb (IV)] group at the E/T ratio