Figure 10.

Effect of IFNγ and CD40L (Comb) gene‐transfection therapy on spleen cells. Spleen was collected from mice with the indicated treatments at 7 days after the second treatment. Mature DCs (MHC II ^high CD83⁺ cells), MDSCs (CD11b⁺, Gr1⁺ cells), NK (NK1.1⁺) cells and PDL‐1⁺ cells in the tumor tissues were detected in FCM. Results are expressed as mean ± SE. The experiment was performed using three mice in each treatment group. All mice were humanely euthanized according to the criteria in Section 2. *P < .05, **P < .01 by the Tukey‐Kramer test