Table 2.
Selected m/z values and collision energy for GalCer and 2‐OH GalCer species in LC‐MS/MS analyses
| Lipid species |
Precursor ions (Q1) [M−H2O+H]+ |
Product ion (Q3) | Collision energy (eV) |
|---|---|---|---|
| C12:0 GalCer* | 626.6 | 264.3 | 40 |
| C16:0 GalCer | 682.6 | 264.3 | 40 |
| C18:1 GalCer | 708.6 | 264.3 | 40 |
| C18:0 GalCer | 710.6 | 264.3 | 40 |
| C20:1 GalCer | 736.6 | 264.3 | 40 |
| C20:0 GalCer | 738.6 | 264.3 | 40 |
| C22:1 GalCer | 764.6 | 264.3 | 40 |
| C22:0 GalCer | 766.6 | 264.3 | 40 |
| C24:1 GalCer | 792.7 | 264.3 | 40 |
| C24:0 GalCer | 794.7 | 264.3 | 40 |
| C26:1 GalCer | 820.7 | 264.3 | 40 |
| C26:0 GalCer | 822.7 | 264.3 | 40 |
| 2‐OH C16:0 GalCer | 698.6 | 264.3 | 40 |
| 2‐OH C18:1 GalCer | 724.6 | 264.3 | 40 |
| 2‐OH C18:0 GalCer | 726.6 | 264.3 | 40 |
| 2‐OH C20:1 GalCer | 752.6 | 264.3 | 40 |
| 2‐OH C20:0 GalCer | 754.6 | 264.3 | 40 |
| 2‐OH C22:1 GalCer | 780.6 | 264.3 | 40 |
| 2‐OH C22:0 GalCer | 782.6 | 264.3 | 40 |
| 2‐OH C24:1 GalCer | 808.7 | 264.3 | 40 |
| 2‐OH C24:0 GalCer | 810.7 | 264.3 | 40 |
| 2‐OH C26:1 GalCer | 836.7 | 264.3 | 40 |
| 2‐OH C26:0 GalCer | 838.7 | 264.3 | 40 |
*
External control